By considering the notion of Golden manifold and natural symplectic form on a generalized tangent bundle, we introduce generalized symplectic Golden structures on manifolds and obtain integrability conditions in terms of bivector fields, 2-forms, 1-forms and endomorphisms on manifolds and investigate isotropic subbundles. We also find certain relations between the integrability conditions of generalized symplectic Golden manifolds and Lie Groupoids which are important in mechanics as configuration space.