We study adjointable, bounded operators on the direct sum of two copies of the standard Hilbert C *-module over a unital C *-algebra A that are given by upper triangular 2 by 2 operator matrices. Using the definition of A-Fredholm and semi-A-Fredholm operators given in [3], [4], we obtain conditions relating semi-A-Fredholmness of these operators and that of their diagonal entries, thus generalizing the results in [1], [2]. Moreover, we generalize the notion of the spectra of operators by replacing scalars by the elements in the C *-algebra A. Considering these new spectra in A of bounded, adjointable operators on Hilbert C *-modules over A related to the classes of A-Fredholm and semi-A-Fredholm operators, we prove an analogue or a generalized version of the results in [1] concerning the relationship between the spectra of 2 by 2 upper triangular operator matrices and the spectra of their diagonal entries